Mila Marinovic

  • University of Helsinki
  • Department of Applied Chemistry and Microbiology
  • Faculty of Agriculture and Forestry
  • Biocenter 1
  • Viikinkaari 9
  • 00014 Finland

 

My SuBiCat publications:

 

2017  Temporal transcriptome analysis of the white-rot fungus Obba rivulosa shows expression of a constitutive set of plant cell wall degradation targeted genes during growth on solid spruce wood

Mila Marinović, Maria Victoria Aguilar-Pontes, Miaomiao Zhou, Otto Miettinen, Ronald P.de Vries, Miia R.Mäkelä, KristiinaHildén, Fungal Genet. Biol. 2017. doi: 10.1016/j.fgb.2017.07.004 In Press

The basidiomycete white-rot fungus Obba rivulosa, a close relative of Gelatoporia (Ceriporiopsis) subvermispora, is an efficient degrader of softwood. The dikaryotic O. rivulosa strain T241i (FBCC949) has been shown to selectively remove lignin from spruce wood prior to depolymerization of plant cell wall polysaccharides, thus possessing potential in biotechnological applications such as pretreatment of wood in pulp and paper industry. In this work, we studied the time-course of the conversion of spruce by the genome-sequenced monokaryotic O. rivulosa strain 3A-2, which is derived from the dikaryon T241i, to get insight into transcriptome level changes during prolonged solid state cultivation. During 8-week cultivation, O. rivulosa expressed a constitutive set of genes encoding putative plant cell wall degrading enzymes. High level of expression of the genes targeted towards all plant cell wall polymers was detected at 2-week time point, after which majority of the genes showed reduced expression. This implicated non-selective degradation of lignin by the O. rivulosa monokaryon and suggests high variation between mono- and dikaryotic strains of the white-rot fungi with respect to their abilities to convert plant cell wall polymers.

 

2017  Selective cleavage of lignin β-O-4 aryl ether bond by β-etherase of the white-rot fungus Dichomitus squalens

Marinović, M., Nousiainen, P., Dilokpimol, A., Kontro, J., Moore, R., Sipilä, J., de Vries, R.P., Mäkelä M.R., Hildén, K., Submitted manuscript to ACS Sustainable Chem. Eng

 

 

My SuBiCat poster presentations:

 

Selective Cleavage of β-O-4 Lignin Bond by Novel β-etherase of the White Rot Fungus Dichomitus squalens

29th Fungal Genetics Conference, Pacific Grove, CA, USA, March 2017

 

Glutathione-S-transferases of the White Rot Fungus Dichomitus squalens: Expression Genes and Production of Proteins

13th European Conference on Fungal Genetics, Paris, France, April 2016

 

Production of lignin-modifying enzymes in selected basidiomycetous white-rot and litter-decomposing fungi

Tailor-Made Fuels from Biomass, 2nd International Conference, Aachen, Germany, June 16th – 18th 2014

 

E-mail: mila.marinovic@helsinki.fi