Selin Ece

 

My SuBiCat publications:

 

2017  Heterologous expression of a Streptomyces cyaneus laccase for biomass modification applications

Ece, S., Lambertz, C., Fischer, R., & Commandeur, U., AMB Express 2017, 7(1), 86

Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing Streptomyces cyaneus CECT 3335 laccase in Escherichia coli (ScLac) and we achieved a yield of up to 104 mg L−1 following purification by one-step affinity chromatography. Stability and activity assays using simple lignin model substrates showed that the purified enzyme preparation was active over a broad pH range and at high temperatures, suggesting it would be suitable for biomass degradation. The reusability of ScLac was also demonstrated by immobilizing the enzyme on agarose beads with a binding yield of 33%, and by the synthesis of cross-linked enzyme aggregates with an initial activity recovery of 72%.

 

2016  Progress and obstacles in the production and application of recombinant lignin-degrading peroxidases

Camilla Lambertz, Selin Ece, Rainer Fischer & Ulrich Commandeur, Bioengineered. 2016 Apr;7(3):145-54

Lignin is 1 of the 3 major components of lignocellulose. Its polymeric structure includes aromatic subunits that can be converted into high-value-added products, but this potential cannot yet been fully exploited because lignin is highly recalcitrant to degradation. Different approaches for the depolymerization of lignin have been tested, including pyrolysis, chemical oxidation, and hydrolysis under supercritical conditions. An additional strategy is the use of lignin-degrading enzymes, which imitates the natural degradation process. A versatile set of enzymes for lignin degradation has been identified, and research has focused on the production of recombinant enzymes in sufficient amounts to characterize their structure and reaction mechanisms. Enzymes have been analyzed individually and in combinations using artificial substrates, lignin model compounds, lignin and lignocellulose. Here we consider progress in the production of recombinant lignin-degrading peroxidases, the advantages and disadvantages of different expression hosts, and obstacles that must be overcome before such enzymes can be characterized and used for the industrial processing of lignin.

My SuBiCat poster presentations:

 

Recombinant production of lignin-degrading enzymes

Tailor-Made Fuels from Biomass, 2nd International Conference, Aachen, Germany, June 16th – 18th 2014

 

Recombinant Expression of a DyP-type Peroxidase in an E. coli Host System for Effective Lignin Degradation

7th International Congress on Biocatalysis (biocat2014), Hamburg, Germany, August 31st – September 4th 2014

 

E-mail: selin.ece@molbiotech.rwth-aachen.de

Direct phone: +49-241-802-8317